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N Use By Plants
Nitrate Assimilation
Ammonia Assimilation
Glu, Gln, Asn, Gly, Ser
Asp, Ala, GABA
Val, Leu, Ileu, Thr, Lys
Pro, Arg, Orn
Non-protein AAs
Sulfate Assimilation
Cys, Met, AdoMet, ACC
His, Phe, Tyr, Tryp
Secondary Products
Onium Compounds
HORT640 - Metabolic Plant Physiology

Ammonia Assimilation and Recycling

Three Dimensional Structure of Glutamine Synthetase

The image(s) below show the crystal structure of the Salmonella typhimurium glutamine synthetase dodecamer [EC] complexed with ADP, Mn2+ and phosphinothricin. The twelve subunits are shown in different colors. ADP and phosphinothricin are shown in white. The 12 identical subunits form two facing hexagonal arrays. Each catalytic site is at an interface between subunits, and is made up of residues from two adjacent subunits. Image(s) below were obtained using the Glaxo Wellcome Experimental Research Swiss-Pdb Viewer (v3.6b3) (see references below), and crystal structure data derived from: Gill HS, Eisenberg D 2001 The crystal structure of phosphinothricin in the active site of glutamine synthetase illuminates the mechanism of enzymatic inhibition. Biochemistry 40: 1903-1912 [sequence = GLNA_SALTY P06201].

    Guex, N., Diemand, A. and Peitsch, M.C. (1999)
    Protein modelling for all.
    TiBS 24: 364-367.

    Guex, N. and Peitsch, M. C. (1997)
    SWISS-MODEL and the Swiss-PdbViewer: An environment for
    comparative protein modelling.
    Electrophoresis 18: 2714-2723.

    Peitsch, M. C. (1995)
    Protein modeling by E-mail
    Bio/Technology 13: 658-660.

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David Rhodes
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Last Update: 10/01/09